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排序方式: 共有236条查询结果,搜索用时 218 毫秒
51.
Christian David Andreas Schmid Lorenz Adrian Annegret Wilde Katja Bühler 《Biotechnology and bioengineering》2018,115(2):300-311
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Biosynthesis of ferulic acid esters of plant cell wall polysaccharides in endomembranes from parsley cells 总被引:3,自引:0,他引:3
A microsomal preparation from suspension-cultured parsley cells is able to transfer ferulic acid from the respective CoA thioester to endogenous acceptors. The reaction is not enhanced by digitonin but stimulated by Mg2+, Ca2+ and Co2+. Spermine can partly replace divalent ions. Solubility properties and degradation by polysaccharide hydrolases suggest that the products are polymeric cell wall carbohydrates. Sucrose density gradient centrifugation revealed that the most active vesicle fraction is distinct from plasma membranes but does also not peak with IDPase. It is suggested that a subfraction of the Golgi-apparatus is the source of enzyme and acceptors. 相似文献
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Belastung des Kiebitzes (Vanellus vanellus) mit Dieldrin und anderen chlorierten Kohlenwasserstoffen
Martin Beyerbach Annegret Büth Walter A. Heidmann Hermann Knüwer und Harald A. Rüssel-Sinn 《Journal of Ornithology》1988,129(3):353-361
Zusammenfassung 45 Eier und 8 Kükenlebern des Kiebitzes aus den Jahren 1984 und 1986 wurden auf -, -, -HCH, HCB, DDT, Dieldrin, Endrin und PCB untersucht. Hohe Konzentrationen an Dieldrin wurden in den Eiern gefunden, während die Lebern von Kiebitzküken geringer belastet waren. Die geringen Konzentrationen der übrigen Pestizide und die mäßige an PCB entsprachen der niedrigen Trophieebene des Kiebitzes. Die Frage, ob die Belastung mit Dieldrin einen Einfluß auf den Rückgang von Kiebitzpopulationen in einigen Gebieten haben könnte, wird kurz diskutiert.
Gefördert mit Hilfe von Forschungsmitteln des Landes Niedersachsen 相似文献
The burden of Dieldrin and other chlorinated hydrocarbons on the Lapwing (Vanellus vanellus)
Summary -, -, -HCH, HCB DDT, Dieldrin, Endrin, and PCB were determined in 45 eggs and 8 livers of young Lapwings in 1984 and 1986. High concentrations of Dieldrin were found in eggs while those in livers of young Lapwings were lower. The low residues of the other pesticides and the moderate ones of PCB are in accordance with the low trophic level of the Lapwing. The probable influence of the Dieldrin burden on the decline of Lapwing populations in several regions is discussed briefly.
Gefördert mit Hilfe von Forschungsmitteln des Landes Niedersachsen 相似文献
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Reisenauer A Eickelberg O Wille A Heimburg A Reinhold A Sloane BF Welte T Bühling F 《Biological chemistry》2007,388(6):639-650
The TGF-beta signaling pathways are implicated in cancer. Cysteine cathepsins can contribute to the carcinogenic potential of tumor cells. The aim of this study was to investigate the regulation of cysteine cathepsin expression by TGF-beta1 and the functional implications in tumor cells. We found an upregulation of cathepsin B (CathB, 2- to 5-fold) in different myeloid tumor cells (THP-1, MonoMac-1, MonoMac-6) after incubation with TGF-beta1. No upregulation was found in monocytes, and there was suppression of CathB expression in epithelial tumor cells (A549). Increased cathepsin B activity led to enhanced carcinogenic potential, which was reflected by increased migration and invasion of the cells and resistance to inhibitor-induced apoptosis. Analysis of the TGF-beta signaling pathways showed no alterations in TGF-beta/BMP receptor expression or SMAD2/3 phosphorylation, and no influence of MAP kinase pathways. However, a reduction in SMAD1 expression was detected. The lack of BMP action on cysteine cathepsin expression in myeloid tumor cells, but not in epithelial tumor cells, suggests a defect in the Smad1/Smad5 pathway. We located a related TGF-beta1-responsive element within the first intron of the CathB gene. In conclusion, alterations in the TGF-beta1 signaling pathway lead to upregulation of CathB, which contributes to the carcinogenic potential of tumor cells. 相似文献
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Stoye D Schubert C Goihl A Guttek K Reinhold A Brocke S Grüngreiff K Reinhold D 《Biometals》2012,25(3):529-539
Zinc is an essential trace element with a critical role in normal growth and development and in immune homeostasis. Zinc deficiency impairs both the innate and the adaptive immune system and can be normalized by zinc supplementation. On the other end of the spectrum, high dosages of zinc diminish immune cell functions similar to zinc deficiency. Here, we investigated the influence of zinc aspartate on proliferation and cytokine production of stimulated human T cells and mouse splenocytes in vitro. Furthermore, the effect of zinc aspartate was examined in mice with experimental autoimmune encephalomyelitis (EAE), an animal model of Multiple Sclerosis (MS) with a Th1/Th17 T cell-mediated immunopathogenesis. Zinc aspartate suppressed proliferation as well as IL-2, IL-10 and IL-17 production in stimulated human T cells and mouse splenocytes. Importantly, administration of a medium range dose of 30 μg/day zinc aspartate [1.5 mg/kg body weight (BW)] in a therapeutic manner led to a significant reduction of the clinical severity of the EAE during the first relapse of the disease. A lower zinc aspartate dose (6 μg/day, 0.3 mg/kg BW) had no significant therapeutic effect on the severity of the EAE, while administration of higher zinc aspartate amounts (120 μg/day, 6 mg/kg BW) led to more severe disease. Taken together, our data suggest that zinc aspartate can modulate activation, proliferation and cytokine production of effector T cells in vitro and in vivo and that activated autoreactive T cells may be potential therapeutic targets of tightly controlled zinc supplementation in autoimmune diseases like MS. 相似文献
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Mutations in the tight-junction gene claudin 19 (CLDN19) are associated with renal magnesium wasting, renal failure, and severe ocular involvement 总被引:8,自引:0,他引:8 下载免费PDF全文
Konrad M Schaller A Seelow D Pandey AV Waldegger S Lesslauer A Vitzthum H Suzuki Y Luk JM Becker C Schlingmann KP Schmid M Rodriguez-Soriano J Ariceta G Cano F Enriquez R Juppner H Bakkaloglu SA Hediger MA Gallati S Neuhauss SC Nurnberg P Weber S 《American journal of human genetics》2006,79(5):949-957
Claudins are major components of tight junctions and contribute to the epithelial-barrier function by restricting free diffusion of solutes through the paracellular pathway. We have mapped a new locus for recessive renal magnesium loss on chromosome 1p34.2 and have identified mutations in CLDN19, a member of the claudin multigene family, in patients affected by hypomagnesemia, renal failure, and severe ocular abnormalities. CLDN19 encodes the tight-junction protein claudin-19, and we demonstrate high expression of CLDN19 in renal tubules and the retina. The identified mutations interfere severely with either cell-membrane trafficking or the assembly of the claudin-19 protein. The identification of CLDN19 mutations in patients with chronic renal failure and severe visual impairment supports the fundamental role of claudin-19 for normal renal tubular function and undisturbed organization and development of the retina. 相似文献
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